Synthesis of dolichyl phosphate derivatives with fluorescent label at the omega-end of the chain, new tools to study protein glycosylation

Bioorg Med Chem Lett. 2000 Jan 17;10(2):189-92. doi: 10.1016/s0960-894x(99)00662-9.

Abstract

Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the omega-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 1-Naphthylamine / chemistry
  • Aminopyridines / chemistry
  • Dolichol Phosphates / chemical synthesis*
  • Fluorescent Dyes / chemical synthesis*
  • Glycoproteins / chemistry*
  • Kinetics
  • Mannosyltransferases / metabolism
  • Substrate Specificity
  • Yeasts / enzymology

Substances

  • Aminopyridines
  • Dolichol Phosphates
  • Fluorescent Dyes
  • Glycoproteins
  • 1-Naphthylamine
  • Mannosyltransferases
  • dolichyl-phosphate beta-D-mannosyltransferase
  • alpha-aminopyridine