Abstract
Derivatives of dolichyl phosphate (Dol-P) with 2-aminopyridine or 1-aminonaphtalene fluorophore groups at the omega-end of the chain were synthesized. These products serve as substrates for recombinant yeast Dol-P-mannose synthase. Fluorescence resonance energy transfer between a Trp residue of the enzyme and the 1-aminonaphtalene group of the Dol-P analogue was demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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1-Naphthylamine / chemistry
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Aminopyridines / chemistry
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Dolichol Phosphates / chemical synthesis*
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Fluorescent Dyes / chemical synthesis*
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Glycoproteins / chemistry*
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Kinetics
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Mannosyltransferases / metabolism
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Substrate Specificity
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Yeasts / enzymology
Substances
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Aminopyridines
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Dolichol Phosphates
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Fluorescent Dyes
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Glycoproteins
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1-Naphthylamine
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Mannosyltransferases
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dolichyl-phosphate beta-D-mannosyltransferase
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alpha-aminopyridine