Abstract
We examined the effect of grepafloxacin (GPFX), a new fluoroquinolone antimicrobial agent, on interleukin-8 (IL-8) expression in tumor necrosis factor-alpha (TNF-alpha)-stimulated human airway epithelial cells (AEC). GPFX inhibited IL-8 protein production as well as mRNA expression in a concentration-dependent manner (2.5 - 25 micro g/ml), but the inhibition of IL-8 expression by corresponding concentrations of GPFX to serum and airway lining fluids was not complete. We discuss the modulatory effect of GPFX on IL-8 production in the context of its efficacy on controlling chronic airway inflammatory diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / pharmacology*
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Cell Survival / drug effects
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Cells, Cultured
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Culture Media, Conditioned / chemistry
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Dose-Response Relationship, Drug
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Fluoroquinolones*
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Humans
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Interleukin-8 / biosynthesis*
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Interleukin-8 / genetics
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Piperazines / pharmacology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Respiratory Mucosa / cytology
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Respiratory Mucosa / drug effects*
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Respiratory Mucosa / metabolism*
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Time Factors
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Tumor Necrosis Factor-alpha / antagonists & inhibitors*
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Tumor Necrosis Factor-alpha / pharmacology*
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Up-Regulation / drug effects
Substances
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Anti-Infective Agents
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Culture Media, Conditioned
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Fluoroquinolones
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Interleukin-8
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Piperazines
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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grepafloxacin