Abstract
Experimental infection of C57BL/6 mice by Plasmodium yoelii sporozoites induced an increase of CD4-CD8- NK1.1+ TCR alpha beta int cells and a down-regulation of CD4+ NK1.1+ TCR alpha beta int cells in the liver during the acute phase of the infection. These cells showed an activated CD69+, CD122+, CD44high, and CD62Lhigh surface phenotype. Analysis of the expressed TCRV beta segment repertoire revealed that most of the expanded CD4-CD8- (double-negative) T cells presented a skewed TCRV beta repertoire and preferentially used V beta 2 and V beta 7 rather than V beta 8. To get an insight into the function of expanded NK1.1+ T cells, experiments were designed in vitro to study their activity against P. yoelii liver stage development. P. yoelii-primed CD3+ NK1.1+ intrahepatic lymphocytes inhibited parasite growth within the hepatocyte. The antiplasmodial effector function of the parasite-induced NK1.1+ liver T cells was almost totally reversed with an anti-CD3 Ab. Moreover, IFN-gamma was in part involved in this antiparasite activity. These results suggest that up-regulation of CD4-CD8- NK1.1+ alpha beta T cells and down-regulation of CD4+ NK1.1+ TCR alpha beta int cells may contribute to the early immune response induced by the Plasmodium during the prime infection.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens / biosynthesis
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Antigens, Ly
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Antigens, Surface
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CD4 Antigens / biosynthesis
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CD8 Antigens / biosynthesis
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Cells, Cultured
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Down-Regulation / immunology
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Gene Expression Regulation / immunology
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Genes, T-Cell Receptor beta
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Lectins, C-Type
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Liver / immunology
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Liver / parasitology*
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Liver / pathology
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Liver Diseases, Parasitic / immunology*
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Liver Diseases, Parasitic / metabolism
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Liver Diseases, Parasitic / parasitology
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Lymphocyte Count
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Malaria / immunology*
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Malaria / metabolism
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Malaria / parasitology*
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Mice
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Mice, Inbred C57BL
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NK Cell Lectin-Like Receptor Subfamily B
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Plasmodium yoelii / growth & development*
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Plasmodium yoelii / immunology*
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Protein Biosynthesis
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Proteins*
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Receptors, Antigen, T-Cell, alpha-beta / biosynthesis
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
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T-Lymphocyte Subsets / parasitology*
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T-Lymphocytes, Regulatory / immunology*
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T-Lymphocytes, Regulatory / metabolism
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T-Lymphocytes, Regulatory / parasitology
Substances
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Antigens
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Antigens, Ly
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Antigens, Surface
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CD4 Antigens
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CD8 Antigens
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Klrb1c protein, mouse
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Lectins, C-Type
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NK Cell Lectin-Like Receptor Subfamily B
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Proteins
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Receptors, Antigen, T-Cell, alpha-beta