Sychnocotyle kholo n. g., n. sp. (Aspidogastrea: Aspidogastridae) is described from the small intestine of the freshwater turtle Emydura macquarii (Pleurodira: Chelidae). The new genus is distinguished from other aspidogastrids by the possession of the following suite of characters: no cirrus-sac; no hermaphroditic duct; four rows of alveoli on the ventral disc but no prominent papillae; Laurer's canal opening to the exterior. Eggs hatched to cotylocidia within 37-41 days at 17-24 degrees C. The life-cycle is obligate two-host, involving a mollusc and a freshwater turtle. Juvenile forms, almost entirely single worm infections, were found in the molluscs Corbiculina sp. (Bivalvia: Corbiculidae) and Thiara balonnensis (Prosobranchia: Thiaridae). Sychnocotyle is most likely to have evolved in Australia after the separation of Gondwanaland from Laurasia 120- 100 mya.