Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods

M Vaïtilingom; H Pijnenburg; F Gendre; P Brignon

doi:10.1021/jf981208v

Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods

J Agric Food Chem. 1999 Dec;47(12):5261-6. doi: 10.1021/jf981208v.

Authors

M Vaïtilingom¹, H Pijnenburg, F Gendre, P Brignon

Affiliation

¹ Tepral, Beverage Division Research Center of Danone Group, 68 Route d'Oberhausbergen, 67037 Strasbourg Cedex, France.

PMID: 10606606
DOI: 10.1021/jf981208v

Abstract

A fast and quantitative method was developed to detect transgenic "Maximizer" maize "event 176" (Novartis) and "Roundup Ready" soybean (Monsanto) in food by real-time quantitative PCR. The use of the ABI Prism 7700 sequence detection system allowed the determination of the amplified product accumulation through a fluorogenic probe (TaqMan). Fluorescent dyes were chosen in such a way as to coamplify total and transgenic DNA in the same tube. Using real-time quantitative PCR, 2 pg of transgenic or total DNA per gram of starting sample was detected in 3 h after DNA extraction and the relative amounts of "Maximizer" maize and "Roundup Ready" soybean in some representative food products were quantified.

MeSH terms

DNA / isolation & purification
DNA Primers
Genetic Engineering
Glycine max / chemistry*
Glycine max / genetics
Humans
Plants, Genetically Modified / chemistry
Plants, Genetically Modified / genetics
Polymerase Chain Reaction
Zea mays / chemistry*
Zea mays / genetics

Substances

DNA Primers
DNA