A systematic approach for fish shelf life modelling and Time Temperature Integrator (TTI) selection in order to plan and apply an effective quality monitoring scheme for the fish chill chain was developed. The temperature behaviour of the natural microflora of the Mediterranean fish boque (Boops boops) was studied and growth of the specific spoilage bacteria Pseudomonas spp. and Shewanella putrefaciens was modelled and correlated to organoleptic shelf life. Arrhenius and square root functions were used to model temperature dependence of maximum growth rates. Bacterial growth and shelf life models were validated under dynamic storage conditions with independent variable temperature experiments. The response of several TTIs from similar storage experiments was also modelled. The reliability of the TTI monitoring was cumulatively expressed by the error in the TTI derived effective temperature (Teff) for different variable temperature distributions. Teff was directly translated to shelf life of the fish.