The use of yeast artificial chromosomes in transgenic animals: expression studies of the tyrosinase gene in transgenic mice

Genet Anal. 1999 Nov;15(3-5):175-8. doi: 10.1016/s1050-3862(99)00023-6.

Abstract

Variegation and inherited somatic mosaicism has been observed in transgenic mice carrying yeast artificial chromosomes (YACs) in which a DNAse I hypersensitive site (HS) located -12 kb upstream of the mouse tyrosinase gene had been deleted. At present, we are generating new transgenic animals with minor deletions of the HS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Chromosomes, Artificial, Yeast*
  • DNA, Recombinant / genetics
  • DNA, Recombinant / metabolism
  • Deoxyribonuclease I / metabolism
  • Genes, Synthetic
  • Hair Color / genetics
  • Mice
  • Mice, Transgenic
  • Monophenol Monooxygenase / biosynthesis
  • Monophenol Monooxygenase / genetics*
  • Mosaicism
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Sequence Deletion
  • Transgenes*

Substances

  • DNA, Recombinant
  • Recombinant Fusion Proteins
  • Monophenol Monooxygenase
  • Deoxyribonuclease I