Abstract
aprX is a 1326 bp gene of Bacillus subtilis strain 168 that encodes a serine protease, probably intracellular, characterized by significant similarity with subtilisins, thermitases and pyrolysins. Transcription analysis, performed by RT-PCR and primer extension, allowed the localization of the active promoter and showed that aprX is expressed in stationary phase. The pattern of expression of aprX and its dependence on various transition state regulatory genes (degU, degQ, hpr, abrB, sinR), monitored by lacZ transcriptional fusions, are distinctive from those of subtilisin and other degradative enzymes. aprX is not essential for either growth or sporulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / genetics*
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Bacterial Proteins*
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Base Sequence
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Consensus Sequence
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Electrophoresis, Agar Gel
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Gene Expression
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Genes, Bacterial / genetics*
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction
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RNA, Bacterial / analysis
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RNA, Messenger / analysis
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Serine Endopeptidases / chemistry
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Serine Endopeptidases / genetics*
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Serine Endopeptidases / metabolism
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Species Specificity
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Subtilisin / classification
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Subtilisin / genetics*
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Subtilisin / metabolism
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Transcription, Genetic
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beta-Galactosidase / metabolism
Substances
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Bacterial Proteins
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RNA, Bacterial
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RNA, Messenger
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beta-Galactosidase
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AprX protein, bacteria
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Serine Endopeptidases
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Subtilisin