Abstract
Using a lac reporter system in Escherichia coli, we showed that the expression of E. herbicola tpl was regulated through TyrR and cAMP receptor protein. Three TyrR boxes upstream of tpl were essential for full expression. The results suggested that the tyrosine-mediated TyrR hexamerization was an important process. The DNA bending between two TyrR boxes, which is triggered by the binding of cAMP receptor protein, may facilitate the conformational change of TyrRs.
MeSH terms
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Artificial Gene Fusion
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Base Sequence
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Cyclic AMP Receptor Protein / metabolism*
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Erwinia / enzymology*
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Erwinia / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins*
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Gene Expression Regulation, Bacterial*
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Glucose / metabolism
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Molecular Sequence Data
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Plasmids / metabolism
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Repressor Proteins / metabolism*
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Tyrosine Phenol-Lyase / genetics*
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Tyrosine Phenol-Lyase / metabolism
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beta-Galactosidase / metabolism
Substances
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Cyclic AMP Receptor Protein
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Escherichia coli Proteins
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Repressor Proteins
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TyrR protein, E coli
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beta-Galactosidase
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Tyrosine Phenol-Lyase
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Glucose