Abstract
Methods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cell Transformation, Neoplastic
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Gene Expression / drug effects
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Half-Life
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Isoenzymes / genetics
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Isoenzymes / metabolism
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JNK Mitogen-Activated Protein Kinases
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Liposomes
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Mice
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Mice, Knockout
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Mitogen-Activated Protein Kinase 10
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Mitogen-Activated Protein Kinase 9
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Mitogen-Activated Protein Kinases / genetics*
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Mitogen-Activated Protein Kinases / metabolism
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Multigene Family*
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Oligonucleotides, Antisense / pharmacology*
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Phenotype
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Protein Kinases / genetics
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Protein Kinases / metabolism
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism
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Thionucleotides / pharmacology
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Transfection
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Transplantation, Heterologous
Substances
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Isoenzymes
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Liposomes
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Oligonucleotides, Antisense
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Thionucleotides
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Protein Kinases
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Mitogen-Activated Protein Kinase 10
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Mitogen-Activated Protein Kinase 9
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Protein-Tyrosine Kinases
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JNK Mitogen-Activated Protein Kinases
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Mitogen-Activated Protein Kinases