Antisense methods for discrimination of phenotypic properties of closely related gene products: Jun kinase family

Methods Enzymol. 2000:314:342-62. doi: 10.1016/s0076-6879(99)14114-4.

Abstract

Methods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Transformation, Neoplastic
  • Gene Expression / drug effects
  • Half-Life
  • Isoenzymes / genetics
  • Isoenzymes / metabolism
  • JNK Mitogen-Activated Protein Kinases
  • Liposomes
  • Mice
  • Mice, Knockout
  • Mitogen-Activated Protein Kinase 10
  • Mitogen-Activated Protein Kinase 9
  • Mitogen-Activated Protein Kinases / genetics*
  • Mitogen-Activated Protein Kinases / metabolism
  • Multigene Family*
  • Oligonucleotides, Antisense / pharmacology*
  • Phenotype
  • Protein Kinases / genetics
  • Protein Kinases / metabolism
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism
  • Thionucleotides / pharmacology
  • Transfection
  • Transplantation, Heterologous

Substances

  • Isoenzymes
  • Liposomes
  • Oligonucleotides, Antisense
  • Thionucleotides
  • Protein Kinases
  • Mitogen-Activated Protein Kinase 10
  • Mitogen-Activated Protein Kinase 9
  • Protein-Tyrosine Kinases
  • JNK Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinases