Since the first human bladder reconstruction in 1989 using an ileal segment, many alternatives have been proposed to recreate a bladder reservoir as adapted as possible to physiological conditions. Since the development of urothelial cell culture alone and then in combination with matricial supports, various experimental trials have studied the possibility of using this neurothelium for surgical purposes. This experimental study in rabbits tested the compatibility of two different biosynthetic supports in an enterocystoplasty and the survival in in vitro urothelial cells grafted onto this support.