Nonaqueous capillary electrophoretic separation of basic enantiomers using heptakis(2,3-dimethyl-6-sulfato)-beta-cyclodextrin

M Tacker; P Glukhovskiy; H Cai; G Vigh

doi:10.1002/(SICI)1522-2683(19990901)20:13<2794::AID-ELPS2794>3.0.CO;2-4

Nonaqueous capillary electrophoretic separation of basic enantiomers using heptakis(2,3-dimethyl-6-sulfato)-beta-cyclodextrin

Electrophoresis. 1999 Sep;20(13):2794-8. doi: 10.1002/(SICI)1522-2683(19990901)20:13<2794::AID-ELPS2794>3.0.CO;2-4.

Authors

M Tacker¹, P Glukhovskiy, H Cai, G Vigh

Affiliation

¹ Department of Chemistry, Texas A&M University, College Station 77842-3012, USA.

Abstract

The enantiomers of 40 basic analytes, mostly pharmaceuticals, were separated by nonaqueous capillary electrophoresis in acidic methanol background electrolytes using the sodium salt of heptakis(2,3-dimethyl-6-sulfato)-beta-cyclodextrin (HDMS-beta-CD). The effective mobilities, separation selectivities, and peak resolution values were determined as a function of the HDMS-beta-CD concentration in the 0-40 mM range and were found to follow the theoretical predictions of the charged resolving agent migration model (CHARM model). Fast, efficient enantiomer separations were achieved for a large number of both very hydrophobic and hydrophilic weak bases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyclodextrins / chemistry*
Electrophoresis, Capillary / methods*
Stereoisomerism
beta-Cyclodextrins*

Substances

Cyclodextrins
beta-Cyclodextrins
heptakis(2,3-dimethyl-6-sulfato)cyclodextrin