Distribution pattern of inhibitory and excitatory synapses in the dendritic tree of single masseter alpha-motoneurons in the cat

Abstract

Little is known about the differences in the distributions of inhibitory and excitatory synapses in the dendritic tree of single motoneurons in the brainstem and spinal cord. In this study, the distribution of gamma-aminobutyric acid (GABA)-, glycine-, and glutamate-like immunoreactivity in axon terminals on dendrites of cat masseter alpha-motoneurons, stained intracellularly with horseradish peroxidase, was examined by using postembedding immunogold histochemistry in serial ultrathin sections. The dendritic tree was divided into three segments: primary (Pd) and distal (Dd) dendrites and intermediate (Id) dendrites between the two segments. Quantitative analysis of 175, 279, and 105 boutons synapsing on 13 Pd, 54 Id, and 81 Dd, respectively, was performed. Fifty percent of the total number of studied boutons were immunopositive for GABA and/or glycine and 48% for glutamate. Among the former, 27% showed glycine immunoreactivity only and 14% were immunoreactive to both glycine and GABA. The remainder (9%) showed immunoreactivity for GABA only. As few as 3% of the boutons were immunonegative for the three amino acids. Most boutons immunoreactive to inhibitory amino acid(s) contained a mixture of spherical, oval, and flattened synaptic vesicles. Most boutons immunoreactive to excitatory amino acid contained clear, spherical, synaptic vesicles with a few dense-cored vesicles. When comparisons of the inhibitory and excitatory boutons were made between the three dendritic segments, the proportion of the inhibitory to the excitatory boutons was high in the Pd (60% vs. 37%) but somewhat low in the Id (46% vs. 52%) and Dd (44% vs. 53%). The percentage of synaptic covering and packing density of the inhibitory synaptic boutons decreased in the order Pd, Id, and Dd, but this trend was not applicable to the excitatory boutons. The present study provides possible evidence that the spatial distribution patterns of inhibitory and excitatory synapses are different in the dendritic tree of jaw-closing alpha-motoneurons.