Induced heteroduplex genotyping of TNF-alpha, IL-1beta, IL-6 and IL-10 polymorphisms associated with transcriptional regulation

Cytokine. 1999 Oct;11(10):789-95. doi: 10.1006/cyto.1999.0491.

Abstract

We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-alpha, IL-1beta, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-alpha positions -308 and -238; IL-1beta position +3953; IL-6 position -174; and IL-10 positions -1082, -819 and -592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Pair Mismatch / genetics
  • Base Sequence
  • Gene Expression Regulation*
  • Gene Frequency
  • Genotype
  • Haplotypes / genetics
  • Heteroduplex Analysis / methods*
  • Humans
  • Interleukin-1 / genetics
  • Interleukin-10 / genetics
  • Interleukin-6 / genetics
  • Interleukins / genetics*
  • Mutagenesis, Site-Directed
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Genetic / genetics*
  • Promoter Regions, Genetic / genetics
  • Transcription, Genetic / genetics*
  • Tumor Necrosis Factor-alpha / genetics*
  • White People / genetics

Substances

  • Interleukin-1
  • Interleukin-6
  • Interleukins
  • Nucleic Acid Heteroduplexes
  • Tumor Necrosis Factor-alpha
  • Interleukin-10