Abstract
We describe the construction and use of 7 induced heteroduplex generators, reagents for the rapid and unequivocal genotyping of nucleotide sequence polymorphism in TNF-alpha, IL-1beta, IL-6 and IL-10. Polymorphisms detected are those previously associated with regulation of gene transcription: TNF-alpha positions -308 and -238; IL-1beta position +3953; IL-6 position -174; and IL-10 positions -1082, -819 and -592. The reagents were used for analysis of allele and haplotype frequencies in a population of healthy Caucasian volunteer blood donors.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Pair Mismatch / genetics
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Base Sequence
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Gene Expression Regulation*
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Gene Frequency
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Genotype
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Haplotypes / genetics
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Heteroduplex Analysis / methods*
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Humans
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Interleukin-1 / genetics
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Interleukin-10 / genetics
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Interleukin-6 / genetics
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Interleukins / genetics*
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Mutagenesis, Site-Directed
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Nucleic Acid Conformation
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Nucleic Acid Heteroduplexes / genetics
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Polymerase Chain Reaction
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Polymorphism, Genetic / genetics*
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Promoter Regions, Genetic / genetics
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Transcription, Genetic / genetics*
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Tumor Necrosis Factor-alpha / genetics*
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White People / genetics
Substances
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Interleukin-1
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Interleukin-6
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Interleukins
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Nucleic Acid Heteroduplexes
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Tumor Necrosis Factor-alpha
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Interleukin-10