Abstract
Nine ciprofloxacin-resistant viridans group streptococci isolated from asymptomatic carriers were analyzed. Identification to the species level by using three different commercial systems and a PCR-based approach was inconsistent. The nucleotide sequences of fragments of the parC, parE, gyrA, and gyrB genes showed considerable intra- and interspecies variations, and these variations mainly involved silent mutations. Three isolates had changes in Ser-79 of ParC (to Phe or Tyr). Phenotypic characterization indicated that eight of the nine isolates had a putative efflux mechanism that would confer low-level resistance to ciprofloxacin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Infective Agents / metabolism
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Anti-Infective Agents / pharmacology*
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biological Transport
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DNA Gyrase
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DNA Topoisomerase IV
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DNA Topoisomerases, Type II / genetics*
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DNA Topoisomerases, Type II / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Drug Resistance, Microbial / genetics
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Fluoroquinolones
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Humans
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Mutation
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Phenotype
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Streptococcus / drug effects*
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Streptococcus / genetics
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Streptococcus / metabolism
Substances
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Anti-Infective Agents
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Bacterial Proteins
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DNA-Binding Proteins
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Fluoroquinolones
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DNA Topoisomerase IV
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DNA Gyrase
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DNA Topoisomerases, Type II
Associated data
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GENBANK/AF144766
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GENBANK/AF144767
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GENBANK/AF144768
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GENBANK/AF144769
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GENBANK/AF144770
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GENBANK/AF144771
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GENBANK/AF144772
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GENBANK/AF144773
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GENBANK/AF144774
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GENBANK/AF144775
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GENBANK/AF144776
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GENBANK/AF144777
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GENBANK/AF144778
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GENBANK/AF144779
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GENBANK/AF144780
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GENBANK/AF144781
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GENBANK/AF144782
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GENBANK/AF144783
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GENBANK/AF144784
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GENBANK/AF144785
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GENBANK/AF144786
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GENBANK/AF144787
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GENBANK/AF144788
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GENBANK/AF144789
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GENBANK/AF144790
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GENBANK/AF144791