A 69 bp fragment in the pyrroline-5-carboxylate reductase promoter of Arabidopsis thaliana activates minimal CaMV 35S promoter in a tissue-specific manner

FEBS Lett. 1999 Sep 17;458(2):193-6. doi: 10.1016/s0014-5793(99)01110-2.

Abstract

The Arabidopsis thaliana gene that encodes pyrroline-5-carboxylate reductase (At-P5R), the last enzyme in proline biosynthesis in A. thaliana, is developmentally regulated and is highly expressed in cells that divide rapidly or undergo changes in osmotic potential. A 69 bp region (P69; -120 to -51) has previously been identified in a 5' deletion analysis of the At-P5R promoter to be necessary for the basal expression. Here, the essential role of P69 for tissue-specific expression of At-P5R is demonstrated by loss- and gain-of-function experiments.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Base Sequence
  • Caulimovirus / genetics*
  • Gene Expression Regulation / physiology
  • Gene Expression Regulation, Plant / physiology*
  • Gene Expression Regulation, Viral / physiology
  • Genes, Plant
  • Genes, Reporter
  • Glucuronidase / biosynthesis
  • Glucuronidase / genetics
  • Molecular Sequence Data
  • Peptide Fragments / genetics*
  • Peptide Fragments / physiology
  • Plants, Genetically Modified
  • Promoter Regions, Genetic* / physiology
  • Pyrroline Carboxylate Reductases / genetics*
  • Pyrroline Carboxylate Reductases / physiology
  • RNA, Ribosomal / genetics*
  • Seeds / genetics
  • Sequence Deletion

Substances

  • Peptide Fragments
  • RNA, Ribosomal
  • Pyrroline Carboxylate Reductases
  • Glucuronidase