Abstract
Bone marrow transplantation with GFP-expressing cells from GFP-transgenic mice resulted in migration of GFP-positive cells into peripheral tissues and brain parenchyma. Most of these cells were observed as colony-like clusters. GFP-positive clusters in the brain were stained by antibody for ER-MP12, but those in the peripheral tissues were not. Since ER-MP12 antigen has been reported as a marker for murine early-stage myeloid precursor, this might suggest that some parts of phagocytic cells in the brain parenchyma such as microglia are derived from undifferentiated pluripotent hematopoietic cells.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biomarkers / analysis
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Bone Marrow Transplantation / physiology*
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Brain / cytology*
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Cell Movement
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Flow Cytometry
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Genes, Reporter
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Green Fluorescent Proteins
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / physiology*
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Liver / cytology
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Luminescent Proteins / genetics*
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Lung / cytology
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Male
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Mesencephalon / physiology
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Mice
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Mice, Inbred Strains
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Mice, Transgenic
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Platelet Endothelial Cell Adhesion Molecule-1 / analysis
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Reverse Transcriptase Polymerase Chain Reaction
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Spleen / cytology
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Transplantation Chimera*
Substances
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Biomarkers
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Luminescent Proteins
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Platelet Endothelial Cell Adhesion Molecule-1
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Green Fluorescent Proteins