Methylobacterium extorquens strain P14, a new methylotrophic bacteria producing poly-beta-hydroxybutyrate (PHB)

Acta Microbiol Pol. 1999;48(1):39-51.

Abstract

Strain P14 of facultative methylotrophic bacteria that synthetisizes poly-beta-hydroxybutyrate has been isolated. The cells are gram-negative motile rods with a polar flagellum. They do not form spores or capsules, but do have a caretenoid pigment. Predominant in the fatty acid composition of the cells is cis-vaccenic acid (cis 18:1: omega 7)--72%. In the phospholipid composition phosphatidylcholine predominates (45%), along with phosphatidylenthanoloamine (27%) and phosphatidylglycerol (17%). The main biquinone is Q-10; other ubiquinones (Q-8, Q-9, Q-11) are present in minor quantities. The cells accomplish the icl-variant of serine pathway. The GC content of DNA (Tm) is 65 mole%. A high level DNA-DNA homology with representatives of the genus Methylobacterium was observed. The strain has been identified as Methylobacterium extorquens strain P14.

MeSH terms

  • Culture Media / chemistry
  • Fatty Acids / analysis
  • Fermentation
  • Gram-Negative Bacteria / classification*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / growth & development
  • Gram-Negative Bacteria / metabolism*
  • Hydrogen-Ion Concentration
  • Hydroxybutyrates / metabolism*
  • Methanol / metabolism*
  • Polyesters / metabolism*

Substances

  • Culture Media
  • Fatty Acids
  • Hydroxybutyrates
  • Polyesters
  • poly-beta-hydroxybutyrate
  • Methanol