Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balance

P Hols; A Ramos; J Hugenholtz; J Delcour; W M de Vos; H Santos; M Kleerebezem

doi:10.1128/JB.181.17.5521-5526.1999

Acetate utilization in Lactococcus lactis deficient in lactate dehydrogenase: a rescue pathway for maintaining redox balance

J Bacteriol. 1999 Sep;181(17):5521-6. doi: 10.1128/JB.181.17.5521-5526.1999.

Authors

P Hols¹, A Ramos, J Hugenholtz, J Delcour, W M de Vos, H Santos, M Kleerebezem

Affiliation

¹ Microbial Ingredients Section, NIZO Food Research and Wageningen Centre for Food Science, 6710 BA Ede, The Netherlands. hols@gene.ucl.ac.be

Abstract

Acetate was shown to improve glucose fermentation in Lactococcus lactis deficient in lactate dehydrogenase. 13C and 1H nuclear magnetic resonance studies using [2-13C]glucose and [2-(13)C]acetate as substrates demonstrated that acetate was exclusively converted to ethanol. This novel pathway provides an alternative route for NAD+ regeneration in the absence of lactate dehydrogenase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetates / metabolism*
Carbon Isotopes
Glucose / metabolism
L-Lactate Dehydrogenase / genetics
L-Lactate Dehydrogenase / metabolism*
Lactococcus lactis / enzymology
Lactococcus lactis / metabolism*
Nuclear Magnetic Resonance, Biomolecular
Oxidation-Reduction
Protons

Substances

Acetates
Carbon Isotopes
Protons
L-Lactate Dehydrogenase
Glucose