Abstract
Monoclonal antibodies generated against multiple antigenic peptides of the N-terminal sequence (3LVPSARAELQSSPLV17) of the cloned delta opioid receptor immunoprecipitated a 58 kDa protein from CHAPS-solubilized NG108-15 membranes. The immunoprecipitates bound [3H]DPDPE--but not [3H]DAMGO--with a Kd of 6.4 nM and a Bmax of 75 pM. Western blot analysis revealed a distinct band of 58 kDa. The antibodies inhibited basal and PGE1-stimulated cAMP levels, and mimicked the effect of agonists manifest in a compensatory increase in cAMP formation. The antibody will be potentially useful in the analysis of functional epitopes on the delta opioid receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenylyl Cyclases / metabolism*
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Alprostadil / pharmacology
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / metabolism*
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Antibodies, Monoclonal / pharmacology
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Blotting, Western
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Cyclic AMP / metabolism
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, D-Penicillamine (2,5)-
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Enkephalins / metabolism
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Epitopes
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Fluorescent Antibody Technique
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Glioma / drug therapy
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Glioma / metabolism
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Molecular Sequence Data
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Neuroblastoma / drug therapy
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Neuroblastoma / metabolism
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Precipitin Tests
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Receptors, Opioid, delta / immunology*
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Tumor Cells, Cultured
Substances
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Antibodies, Monoclonal
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Enkephalins
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Epitopes
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Receptors, Opioid, delta
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Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
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Enkephalin, D-Penicillamine (2,5)-
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Cyclic AMP
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Adenylyl Cyclases
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Alprostadil