Monoclonal antibody to the delta opioid receptor acts as an agonist in dual regulation of adenylate cyclase in NG108-15 cells

FEBS Lett. 1999 Jul 30;456(1):126-30. doi: 10.1016/s0014-5793(99)00878-9.

Abstract

Monoclonal antibodies generated against multiple antigenic peptides of the N-terminal sequence (3LVPSARAELQSSPLV17) of the cloned delta opioid receptor immunoprecipitated a 58 kDa protein from CHAPS-solubilized NG108-15 membranes. The immunoprecipitates bound [3H]DPDPE--but not [3H]DAMGO--with a Kd of 6.4 nM and a Bmax of 75 pM. Western blot analysis revealed a distinct band of 58 kDa. The antibodies inhibited basal and PGE1-stimulated cAMP levels, and mimicked the effect of agonists manifest in a compensatory increase in cAMP formation. The antibody will be potentially useful in the analysis of functional epitopes on the delta opioid receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenylyl Cyclases / metabolism*
  • Alprostadil / pharmacology
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / metabolism*
  • Antibodies, Monoclonal / pharmacology
  • Blotting, Western
  • Cyclic AMP / metabolism
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • Enkephalin, D-Penicillamine (2,5)-
  • Enkephalins / metabolism
  • Epitopes
  • Fluorescent Antibody Technique
  • Glioma / drug therapy
  • Glioma / metabolism
  • Molecular Sequence Data
  • Neuroblastoma / drug therapy
  • Neuroblastoma / metabolism
  • Precipitin Tests
  • Receptors, Opioid, delta / immunology*
  • Tumor Cells, Cultured

Substances

  • Antibodies, Monoclonal
  • Enkephalins
  • Epitopes
  • Receptors, Opioid, delta
  • Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
  • Enkephalin, D-Penicillamine (2,5)-
  • Cyclic AMP
  • Adenylyl Cyclases
  • Alprostadil