Interplay between nitric oxide and CGRP by capsaicin in isolated guinea-pig heart

Pharmacol Res. 1999 Aug;40(2):125-8. doi: 10.1006/phrs.1999.0475.

Abstract

Capsaicin at a concentration of 10(-7)m induced a significant increase in heart rate and increased coronary flow in isolated Langendorff-perfused guinea-pig hearts. This effect was completely blocked by 30 microm of N(omega)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthase. Additional incubation with 3 m m L-Arg antagonized the inhibitory effect of L-NAME. In the presence of 1 microm of a human calcitonin gene-related peptide fragment (hCGRP 8-37), a CGRP-receptor antagonist, L-Arg was without effect. We conclude that a capsaicin-induced increase in coronary flow and heart rate is dependent from an interplay between CGRP and NO in guinea-pig hearts. 1999 Academic Press.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arginine / pharmacology
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Calcitonin Gene-Related Peptide Receptor Antagonists
  • Capsaicin / pharmacology*
  • Coronary Circulation / drug effects
  • Enzyme Inhibitors / pharmacology
  • Guinea Pigs
  • Heart / drug effects*
  • Heart / physiology
  • Heart Rate / drug effects
  • Humans
  • In Vitro Techniques
  • Male
  • NG-Nitroarginine Methyl Ester / pharmacology
  • Nitric Acid / metabolism*

Substances

  • Calcitonin Gene-Related Peptide Receptor Antagonists
  • Enzyme Inhibitors
  • Nitric Acid
  • Arginine
  • Calcitonin Gene-Related Peptide
  • Capsaicin
  • NG-Nitroarginine Methyl Ester