Hydrolysis of some mRNA 5'-cap analogs catalyzed by the human Fhit protein--and lupin ApppA hydrolases

E Bojarska; R Kraciuk; J Wierzchowski; Z Wieczorek; J Stepiński; M Jankowska; E Starzyńska; A Guranowski; E Darzynkiewicz

doi:10.1080/15257779908041666

Hydrolysis of some mRNA 5'-cap analogs catalyzed by the human Fhit protein--and lupin ApppA hydrolases

Nucleosides Nucleotides. 1999 Apr-May;18(4-5):1125-6. doi: 10.1080/15257779908041666.

Authors

E Bojarska¹, R Kraciuk, J Wierzchowski, Z Wieczorek, J Stepiński, M Jankowska, E Starzyńska, A Guranowski, E Darzynkiewicz

Affiliation

¹ Department of Biophysics, University of Warsaw, Poland.

PMID: 10432746
DOI: 10.1080/15257779908041666

Abstract

Hydrolysis of the following four cap analogs: m7G(5')ppp(5')A, m7G(5')ppp(5')m6A, m7G(5')ppp(5')m2'OG and m7G(5')ppp(5')2'dG catalyzed by homogeneous human Fhit protein and yellow lupin Ap3A hydrolase has been investigated. The hydrolysis products were identified by HPLC analysis and the K(m) and Vmax values calculated based on the data obtained by the fluorimetric method.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acid Anhydride Hydrolases / metabolism*
Catalysis
Chromatography, High Pressure Liquid
Humans
Neoplasm Proteins*
Plants / enzymology
Proteins / metabolism*
RNA Caps*
RNA, Messenger / chemistry
RNA, Messenger / metabolism*
Recombinant Proteins / metabolism
Spectrometry, Fluorescence

Substances

Neoplasm Proteins
Proteins
RNA Caps
RNA, Messenger
Recombinant Proteins
fragile histidine triad protein
Acid Anhydride Hydrolases