Scopoletin: an inducible nitric oxide synthesis inhibitory active constituent from Artemisia feddei

Planta Med. 1999 Jun;65(5):400-3. doi: 10.1055/s-1999-14014.

Abstract

Bioassay-guided fractionation of an H2O extract of Artemisia feddei has furnished an inducible nitric oxide synthase (iNOS) inhibitory coumarin, scopoletin (1) and one of the inactive sesquiterpenes, achillin (2). Compound 1 showed inhibition of nitric oxide (NO) synthesis in a dose-dependent manner in murine macrophage-like RAW 264.7 cells stimulated with interferon-gamma (IFN-gamma) plus lipopolysaccharide (LPS). The inhibition of NO synthesis of 1 was due to suppression of iNOS mRNA and iNOS protein, as determined by Northern and Western blotting, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artemisia*
  • Cell Line
  • Cell Survival / drug effects
  • Interferon-gamma / pharmacology
  • Lipopolysaccharides / pharmacology
  • Macrophages
  • Mice
  • Nitric Oxide Synthase / antagonists & inhibitors*
  • Nitric Oxide Synthase / genetics*
  • Nitric Oxide Synthase Type II
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plants, Medicinal*
  • Recombinant Proteins
  • Scopoletin / isolation & purification
  • Scopoletin / pharmacology*

Substances

  • Lipopolysaccharides
  • Plant Extracts
  • Recombinant Proteins
  • Interferon-gamma
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Scopoletin