A monoclonal antibody that recognizes the predicted tick-borne encephalitis virus E protein fusion sequence blocks fusion

T D Volkova; M F Vorovitch; V T Ivanov; A V Timofeev; O M Volpina

doi:10.1007/s007050050566

A monoclonal antibody that recognizes the predicted tick-borne encephalitis virus E protein fusion sequence blocks fusion

Arch Virol. 1999;144(5):1035-9. doi: 10.1007/s007050050566.

Authors

T D Volkova¹, M F Vorovitch, V T Ivanov, A V Timofeev, O M Volpina

Affiliation

¹ Shimyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Moscow, Russia.

PMID: 10416385
DOI: 10.1007/s007050050566

Abstract

The fusion motif of tick-borne encephalitis virus E protein has been predicted to be located within its conserved region (98-120). Results are presented to demonstrate that non-neutralizing monoclonal antibody which recognizes a synthetic peptide corresponding to residues 98-113 of the E protein sequence can block the fusion of the virus particles with artificial membranes.

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal
Antibody Specificity
Antigens, Viral / immunology
Antigens, Viral / physiology
Conserved Sequence
Encephalitis Viruses, Tick-Borne / immunology
Encephalitis Viruses, Tick-Borne / physiology*
Enzyme-Linked Immunosorbent Assay
Horses
Immunoglobulin G
Membrane Fusion / physiology*
Membranes, Artificial
Mice
Molecular Sequence Data
Peptide Fragments / chemistry
Peptide Fragments / immunology
Viral Envelope Proteins / chemistry
Viral Envelope Proteins / immunology
Viral Envelope Proteins / physiology*

Substances

Antibodies, Monoclonal
Antigens, Viral
Immunoglobulin G
Membranes, Artificial
Peptide Fragments
Viral Envelope Proteins
glycoprotein E, Flavivirus