Abstract
We have studied the effects of dietary depletion of vitamin E and selenium on endogenous ubiquinone-dependent antioxidant system. Deficiency induced an increase in both coenzyme Q9 and Q10 in liver tissue, reaching a maximum between 4 and 7 weeks of deficient diet consumption. Cytochrome b5 reductase polypeptide was also enriched in membranes after 5 weeks of deficient diet consumption. Substantial DT-diaphorase activity was found in deficient, but not in control plasma membranes. Deficient membranes were very sensitive to lipid peroxidation, although a great protection was observed after incubation with NAD(P)H. Our results show that liver cells can boost endogenous ubiquinone-dependent protective mechanisms in response to deficiency in vitamin E and selenium.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Membrane / drug effects
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Cell Membrane / metabolism*
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Coenzymes
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Cytochrome Reductases / metabolism
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Cytochrome-B(5) Reductase
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Dihydrolipoamide Dehydrogenase / metabolism
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Electron Transport
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Lipid Peroxidation / drug effects
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Liver / drug effects
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Liver / metabolism*
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Male
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NAD / metabolism
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NADP / metabolism
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Rats
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Rats, Long-Evans
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Selenium / deficiency*
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Selenium / metabolism
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Selenium / pharmacology
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Time Factors
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Ubiquinone / analogs & derivatives
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Ubiquinone / metabolism*
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Vitamin E / metabolism*
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Vitamin E / pharmacology
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Vitamin E Deficiency / metabolism*
Substances
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Coenzymes
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NAD
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Ubiquinone
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Vitamin E
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NADP
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Cytochrome Reductases
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Cytochrome-B(5) Reductase
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Dihydrolipoamide Dehydrogenase
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coenzyme Q10
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Selenium
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ubiquinone 9