Abstract
The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoassay. The anti-HSV-1 MAb showed sensitivity and specificity of 100%, and the anti-HSV-2 MAb showed a sensitivity of 99.46% and 100% specificity, indicating that these MAbs are suitable for typing of clinical HSV isolates.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal / immunology
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Antibodies, Viral* / immunology
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Herpes Genitalis / blood
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Herpes Genitalis / diagnosis*
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Herpes Genitalis / virology
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Herpes Simplex / blood
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Herpes Simplex / diagnosis*
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Herpes Simplex / virology
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Herpesvirus 1, Human / immunology
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Herpesvirus 1, Human / isolation & purification*
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Herpesvirus 2, Human / immunology
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Herpesvirus 2, Human / isolation & purification*
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Humans
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Immunoassay / methods
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Serotyping
Substances
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Antibodies, Monoclonal
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Antibodies, Viral