Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA

N Lemaitre; W Sougakoff; C Truffot-Pernot; V Jarlier

doi:10.1128/AAC.43.7.1761

Characterization of new mutations in pyrazinamide-resistant strains of Mycobacterium tuberculosis and identification of conserved regions important for the catalytic activity of the pyrazinamidase PncA

Antimicrob Agents Chemother. 1999 Jul;43(7):1761-3. doi: 10.1128/AAC.43.7.1761.

Authors

N Lemaitre¹, W Sougakoff, C Truffot-Pernot, V Jarlier

Affiliation

¹ Laboratoire de Recherche Moléculaire sur les Antibiotiques, Faculté de Médecine Pitié-Salpêtrière, Université Pierre et Marie Curie, Paris, France.

Abstract

A new set of mutations, including transposition of the insertion sequence IS6110, was identified in the pncA gene from 19 pyrazinamide-resistant Mycobacterium tuberculosis strains. Alignment of the PncA protein from M. tuberculosis with homologous proteins from different bacterial species revealed three highly conserved regions in PncA which may play an important role in the processing of pyrazinamide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amidohydrolases / chemistry*
Amidohydrolases / physiology
Amino Acid Sequence
Antitubercular Agents / pharmacology*
Catalytic Domain*
Molecular Sequence Data
Mutation*
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Pyrazinamide / metabolism
Pyrazinamide / pharmacology*
Structure-Activity Relationship

Substances

Antitubercular Agents
Pyrazinamide
Amidohydrolases
pyrazinamide deamidase