Molecular cloning and characterization of a locus responsible for O acetylation of the O polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide

C H Zou; Y A Knirel; J H Helbig; U Zähringer; C S Mintz

doi:10.1128/JB.181.13.4137-4141.1999

Molecular cloning and characterization of a locus responsible for O acetylation of the O polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide

J Bacteriol. 1999 Jul;181(13):4137-41. doi: 10.1128/JB.181.13.4137-4141.1999.

Authors

C H Zou¹, Y A Knirel, J H Helbig, U Zähringer, C S Mintz

Affiliation

¹ Department of Microbiology and Immunology, University of Miami School of Medicine, Miami, Florida, 33101, USA.

Abstract

Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide.

MeSH terms

Acetylation
Acetyltransferases
Bacterial Proteins
Carbohydrate Sequence
Cloning, Molecular
Genes, Bacterial*
Genetic Complementation Test
Legionella pneumophila / classification
Legionella pneumophila / genetics*
Molecular Sequence Data
Mutation
Nuclear Magnetic Resonance, Biomolecular
O Antigens / biosynthesis*
O Antigens / chemistry
Peptides / genetics*
Polysaccharides / metabolism
Sequence Analysis
Serotyping

Substances

Bacterial Proteins
O Antigens
Peptides
Polysaccharides
lag-1 protein, Legionella
Acetyltransferases

Associated data

GENBANK/U32118