Abstract
A varriant of competitive ELISA, making it possible to detect fumonisin B1 (up to 0.4 ng/well) was developed, based on the use of specific polyclonal antibodies obtained by immunization of rabbits with a horseradish peroxidase conjugate of fumonisin B1. The minimum concentration of fumonisin B in water-acetonitrile extracts of corn grain, dry corn products, and feeds, detected by the ELISA version developed is 0.2 mg/kg.
MeSH terms
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Animals
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Antibodies / immunology
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Antibody Specificity
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Carboxylic Acids / analysis*
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Carboxylic Acids / immunology
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Carcinogens, Environmental / analysis*
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Cattle
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Enzyme-Linked Immunosorbent Assay / methods*
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Food Microbiology*
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Fumonisins*
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Mycotoxins / analysis*
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Peroxidases
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Rabbits
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Serum Albumin, Bovine
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Zea mays
Substances
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Antibodies
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Carboxylic Acids
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Carcinogens, Environmental
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Fumonisins
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Mycotoxins
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Serum Albumin, Bovine
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fumonisin B1
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Peroxidases