Dried blood specimens spotted on filter paper were evaluated by the HIV-1 DAVIH Dot system from DAVIH Laboratories (Havana, Cuba). The samples used, 103 positive and 105 negative, were previously confirmed by DAVIH Blot from DAVIH Laboratories. In order to use the dried blood on filter paper some modifications were made to the original procedure regarding the times of incubation of the samples and conjugate, and the agitation in both stages. Relative sensitivity and specificity of 100 and 99.05%, respectively, were attained.