Synthesis and properties of (6,7-dimethoxy-4-coumaryl)alanine: a fluorescent peptide label

Anal Biochem. 1999 May 15;270(1):15-23. doi: 10.1006/abio.1999.4081.

Abstract

The synthesis of racemic and l-(6,7-dimethoxy-4-coumaryl)alanine (Dmca) is described and some spectral and physicochemical properties are reported. The utility of Dmca as a highly sensitive and specific label for the quantitative detection of synthetic peptides in HPLC and in minimal essential media (MEM) is also described.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / analogs & derivatives*
  • Alanine / chemical synthesis
  • Alanine / chemistry
  • Coumarins / chemical synthesis*
  • Coumarins / chemistry*
  • Fluorescent Dyes / chemical synthesis*
  • Fluorescent Dyes / chemistry
  • Isomerism
  • Models, Chemical
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet

Substances

  • (6,7-dimethoxy-4-coumaryl)alanine
  • Coumarins
  • Fluorescent Dyes
  • Alanine