To investigate the relationship between mitochondrial enzyme activities and mitochondrial function after cervical cord injury, cervical cord model was prepared using Allen method in cats. Changes in mitochondrial Na+, K(+)-ATPase, Ca2+, Mg(2+)-ATPase, superoxide dismutase (SOD) activities, contents of malondialdehyde (MDA) and mitochondrial function in cervical cord were observed by different biochemical methods. The results showed that mitochondrial Na+, K(+)-ATPase, CA2+, Mg(2+)-ATPase, SOD activities decreased; mitichondral respiratory control rate (RCR), P/O ratio and oxidative phosphorylation rate (OPR) were also depressed is compared with control group (P < 0.05), but MDA level significantly increased compared to control group (P < 0.01). The results demonstrate that there is close correlation, between mitochondrial ATPase. SOD activities and mitochondral function, suggesting that mitochondrial pathophysiological alteration plays important roles in the secondary damage after cervical cord injury.