Sigmoid model versus median-effect analysis for obtaining dose-response curves for in vitro chemosensitivity testing

Int J Clin Pharmacol Ther. 1999 Apr;37(4):189-92.

Abstract

Aim: The aim of this study was to determine how the in vitro dose-response effects of chemotherapeutic agents should be analyzed and reported.

Methods: We arbitrarily evaluated the effects of mitomycin-C and 4-OH-cyclophosphamide on the human ovarian cancer cell line CAOV-3. Dose-response curves (DRCs) were calculated by non-linear (sigmoid model: SigmaPlot) and linear curve fitting (median-effect analysis: CalcuSyn).

Results: In theory and practice, the sigmoid model provided better curve fits and graphical presentation than the median-effect analysis.

Conclusion: Thus, this model should preferably be used as a basis for selection of anti-cancer drugs for clinical use.

Publication types

  • Comparative Study

MeSH terms

  • Antibiotics, Antineoplastic / administration & dosage*
  • Cyclophosphamide / administration & dosage
  • Cyclophosphamide / analogs & derivatives*
  • Dose-Response Relationship, Drug*
  • Female
  • Humans
  • Linear Models
  • Mitomycin / administration & dosage*
  • Models, Statistical*
  • Ovarian Neoplasms / drug therapy*
  • Tumor Cells, Cultured

Substances

  • Antibiotics, Antineoplastic
  • 4-hydroxycyclophosphamide
  • Mitomycin
  • Cyclophosphamide