Abstract
Antioxidant enzymes in liver and small intestine were investigated using control and streptozotocin diabetic rats fed diets with 5% olive, sunflower or fish oil for five weeks. In liver, Glutathione Peroxidase and Superoxide Dismutase decreased and in intestine Glutathione-S-transferase (GST) increased by diabetes. In isolated jejunum and ileum, this increase in GST activity was due to an increase in GST-alpha and -mu isoenzymes in jejunum and GST-alpha, mu and -pi in ileum. Since GST plays an important role in protecting tissues from oxidative damage, our results highlight the role of the intestine against free radicals in physiological or pathological situations.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / metabolism*
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Blood Glucose / metabolism
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Blotting, Western
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Diabetes Mellitus, Experimental / enzymology*
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Dietary Fats / pharmacology*
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Dietary Fats, Unsaturated / pharmacology
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Fatty Acids / pharmacology
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Female
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Fish Oils / pharmacology
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Glutathione Peroxidase / metabolism
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Glutathione Transferase / metabolism*
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Intestines / drug effects
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Intestines / enzymology*
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Isoenzymes / metabolism
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Liver / drug effects
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Liver / enzymology*
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Olive Oil
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Plant Oils / pharmacology
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Rats
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Rats, Wistar
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Sunflower Oil
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Superoxide Dismutase / metabolism
Substances
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Antioxidants
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Blood Glucose
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Dietary Fats
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Dietary Fats, Unsaturated
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Fatty Acids
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Fish Oils
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Isoenzymes
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Olive Oil
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Plant Oils
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Sunflower Oil
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Glutathione Peroxidase
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Superoxide Dismutase
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Glutathione Transferase