Modulation of hepatic and intestinal glutathione S-transferases and other antioxidant enzymes by dietary lipids in streptozotocin diabetic rats

Chemosphere. 1999 Jun;38(13):3003-13. doi: 10.1016/s0045-6535(98)00502-5.

Abstract

Antioxidant enzymes in liver and small intestine were investigated using control and streptozotocin diabetic rats fed diets with 5% olive, sunflower or fish oil for five weeks. In liver, Glutathione Peroxidase and Superoxide Dismutase decreased and in intestine Glutathione-S-transferase (GST) increased by diabetes. In isolated jejunum and ileum, this increase in GST activity was due to an increase in GST-alpha and -mu isoenzymes in jejunum and GST-alpha, mu and -pi in ileum. Since GST plays an important role in protecting tissues from oxidative damage, our results highlight the role of the intestine against free radicals in physiological or pathological situations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Blood Glucose / metabolism
  • Blotting, Western
  • Diabetes Mellitus, Experimental / enzymology*
  • Dietary Fats / pharmacology*
  • Dietary Fats, Unsaturated / pharmacology
  • Fatty Acids / pharmacology
  • Female
  • Fish Oils / pharmacology
  • Glutathione Peroxidase / metabolism
  • Glutathione Transferase / metabolism*
  • Intestines / drug effects
  • Intestines / enzymology*
  • Isoenzymes / metabolism
  • Liver / drug effects
  • Liver / enzymology*
  • Olive Oil
  • Plant Oils / pharmacology
  • Rats
  • Rats, Wistar
  • Sunflower Oil
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Blood Glucose
  • Dietary Fats
  • Dietary Fats, Unsaturated
  • Fatty Acids
  • Fish Oils
  • Isoenzymes
  • Olive Oil
  • Plant Oils
  • Sunflower Oil
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Transferase