Results of electron microscopy-based three-dimensional reconstructions of macromolecules or their complexes are usually stored as density maps. Each point ("voxel") in the map represents a density value and one approach for studying details of the map is to display an isosurface enclosing areas of interest. We have taken a data mining approach not only focusing on the areas of immediate interest but determining all possible separate entities ("blobs") from a density map. After the entire density map is analyzed with our mining program BLOBBER, properties of all detected blobs can be browsed and sets of blobs can be visualized using our VIZBLOB program. Since BLOBBER analyzes density maps using only density information and relates it to spatial relationships, BLOBBER can be used to analyze symmetrical or asymmetrical density maps from any source. To test our program we have analyzed published bacteriophage PRD1 reconstructions. We identified various structural details ranging from individual proteins to major complexes such as the whole capsid shell and more elaborate details of possible connections between membrane interfaces. This approach can also be a useful preprocessing tool for visualizing reconstructions.
Copyright 1999 Academic Press.