A new solid-phase synthesis of oligonucleotides 3'-conjugated with peptides

L De Napoli; A Messere; D Montesarchio; G Piccialli; E Benedetti; E Bucci; F Rossi

doi:10.1016/s0968-0896(98)00250-8

A new solid-phase synthesis of oligonucleotides 3'-conjugated with peptides

Bioorg Med Chem. 1999 Feb;7(2):395-400. doi: 10.1016/s0968-0896(98)00250-8.

Authors

L De Napoli¹, A Messere, D Montesarchio, G Piccialli, E Benedetti, E Bucci, F Rossi

Affiliation

¹ Dipartimento di Chimica Organica e Biologica, Università degli Studi di Napoli Federico II, Italy.

PMID: 10218834
DOI: 10.1016/s0968-0896(98)00250-8

Abstract

A convenient 'on line' solid-phase synthesis of oligonucleotides conjugated at the 3'-end with peptides by means of a polymeric support linking the first nucleoside via the base has been developed. A 17-mer designed for antisense experiments against HIV-1, linking at the 3'-terminus the tripeptide Gly-Gly-His, was prepared in good yields and characterized by MALDI-TOF mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chemistry / methods*
HIV-1 / drug effects
Magnetic Resonance Spectroscopy
Models, Chemical
Oligonucleotides / chemical synthesis*
Peptide Biosynthesis*
Temperature

Substances

Oligonucleotides