Localization of Escherichia coli rpoC mutations that affect RNA polymerase assembly and activity at high temperature

J Bacteriol. 1999 Apr;181(8):2663-5. doi: 10.1128/JB.181.8.2663-2665.1999.

Abstract

We localized five rpoC (beta') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed beta' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved beta' residues participate in interactions holding RNA polymerase together.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Conserved Sequence
  • DNA-Directed RNA Polymerases / biosynthesis*
  • DNA-Directed RNA Polymerases / genetics*
  • DNA-Directed RNA Polymerases / metabolism
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Molecular Sequence Data
  • Point Mutation*
  • Protein Binding
  • Sequence Homology, Amino Acid

Substances

  • beta' subunit of RNA polymerase
  • DNA-Directed RNA Polymerases