Assembly requirements and role of CotH during spore coat formation in Bacillus subtilis

J Bacteriol. 1999 Apr;181(8):2631-3. doi: 10.1128/JB.181.8.2631-2633.1999.

Abstract

We report Western blot data showing that the 42.8-kDa product of the previously characterized cotH locus (8) is a structural component of the Bacillus subtilis spore coat. We show that the assembly of CotH requires both CotE and GerE. In agreement with these observations, the ultrastructural analysis of purified spores suggests that CotH is needed for proper formation of both inner and outer layers of the coat.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacillus subtilis / physiology*
  • Bacillus subtilis / ultrastructure
  • Bacterial Proteins / analysis
  • Bacterial Proteins / metabolism*
  • Cell Wall / chemistry
  • Sigma Factor*
  • Spores, Bacterial / physiology
  • Spores, Bacterial / ultrastructure
  • Transcription Factors*

Substances

  • Bacterial Proteins
  • CotE protein, Bacillus subtilis
  • CotH protein, Bacillus subtilis
  • Sigma Factor
  • Transcription Factors
  • spoIIR protein, Bacillus subtilis
  • spore-specific proteins, Bacillus