The expression of Fos-like immunoreactivity has been studied in spinal segments L5-S1 of decerebrated, unanaesthetized, but otherwise unstimulated rabbits. The aim of the study was to establish baseline levels of Fos in such preparations, and to examine how these might change after spinalization and opioid receptor blockade. In animals with an intact spinal cord, approximately 30 Fos-positive profiles per section were found in the superficial dorsal horns (i.e. laminae I and II) of each 40-microm section, while about 20 profiles per section were found immediately adjacent to the central canal (lamina X). Fos-like immunoreactive profiles were rare elsewhere in the gray matter. When the spinal cord was sectioned at L1 (after blockade with local anaesthetic), significantly more Fos-like immunoreactivity was found in superficial and central regions of the gray matter (approximately 90 profiles per section) in animals perfused 4 h after decerebration, but not when perfusion was performed 2 or 8 h after decerebration. The opioid antagonist naloxone (0.25 mg/kg/h) had little effect on expression of Fos-like immunoreactivity in spinalized preparations, but significantly increased the numbers of Fos-positive profiles in all but the ventral areas of the spinal gray matter in non-spinalized preparations. The present data show that spinal section induces a transient increase in expression of Fos in the superficial and central parts of the spinal gray matter. It appears that spinalization induces spontaneous activity in some neurons in these regions of the cord, presumably as a result of relief of descending inhibition. The effects of naloxone indicate that endogenous opioids exert tonic inhibition over Fos-expressing spinal neurons in non-spinalized rabbits.