Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes

S Hernández-Allés; V J Benedí; L Martínez-Martínez; A Pascual; A Aguilar; J M Tomás; S Albertí

doi:10.1128/AAC.43.4.937

Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes

Antimicrob Agents Chemother. 1999 Apr;43(4):937-9. doi: 10.1128/AAC.43.4.937.

Authors

S Hernández-Allés¹, V J Benedí, L Martínez-Martínez, A Pascual, A Aguilar, J M Tomás, S Albertí

Affiliation

¹ Departamento de Biología, Universidad de las Islas Baleares, Palma de Mallorca, Spain.

Abstract

We have demonstrated by using an in vitro approach that interruption of the OmpK36 porin gene by insertion sequences (ISs) is a common type of mutation that causes loss of porin expression and increased resistance to cefoxitin in Klebsiella pneumoniae. This mechanism also operates in vivo: of 13 porin-deficient cefoxitin-resistant clinical isolates of K. pneumoniae, 4 presented ISs in their ompK36 gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins*
Cefoxitin / pharmacology
Cephamycins / pharmacology
DNA Transposable Elements / genetics
Drug Resistance, Microbial / genetics
Humans
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / metabolism
Mutagenesis, Insertional
Porins / biosynthesis
Porins / genetics*

Substances

Bacterial Proteins
Cephamycins
DNA Transposable Elements
OmpK36 protein, Klebsiella pneumoniae
Porins
Cefoxitin