Characterization of a hexokinase encoding cDNA of the parasitic nematode Hhaemonchus contortus

H P Schmitt-Wrede; A Waldraff; J Krücken; A Harder; F Wunderlich

doi:10.1016/s0167-4781(99)00009-3

Characterization of a hexokinase encoding cDNA of the parasitic nematode Hhaemonchus contortus

Biochim Biophys Acta. 1999 Mar 19;1444(3):439-44. doi: 10.1016/s0167-4781(99)00009-3.

Authors

H P Schmitt-Wrede¹, A Waldraff, J Krücken, A Harder, F Wunderlich

Affiliation

¹ Division of Molecular Parasitology and Centre for Biological and Medical Research, Heinrich-Heine-University, Universitätsstr. 1, 40225, Düsseldorf, Germany.

PMID: 10095069
DOI: 10.1016/s0167-4781(99)00009-3

Abstract

The nematode Haemonchus contortus is an important parasite of cattle and sheep. We describe here the cloning of a cDNA encoding a 53 kDa hexokinase (EC 2.7.1.1). The deduced protein shows 73% identity to a 50 kDa hexokinase deduced from a Caenorhabditis elegans cosmid. Alignment with mammalian hexokinases reveals two short amino acid insertions in the H. contortus hexokinase. Software tools for structural protein analysis (ExPASy server, Geneva) localize these insertions on the surface of the molecule, suggesting these surface changes as potential target sites for chemotherapeutic drugs.

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
DNA, Complementary / chemistry
Haemonchus / genetics*
Hexokinase / genetics*
Molecular Sequence Data
Sequence Alignment

Substances

DNA, Complementary
Hexokinase

Associated data

GENBANK/AJ009635