The NAD+ precursors, nicotinic acid and nicotinamide upregulate glyceraldehyde-3-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase mRNA in Jurkat cells

Biochem Biophys Res Commun. 1999 Feb 5;255(1):133-6. doi: 10.1006/bbrc.1999.0154.

Abstract

To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (G6PD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Gene Expression Regulation, Enzymologic / drug effects
  • Glucosephosphate Dehydrogenase / biosynthesis*
  • Glucosephosphate Dehydrogenase / genetics
  • Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
  • Humans
  • Jurkat Cells
  • NAD / chemistry
  • NAD / pharmacology
  • Niacin / pharmacology*
  • Niacinamide / pharmacology*
  • Oxidative Stress
  • Protein Precursors / chemistry
  • Protein Precursors / pharmacology*
  • RNA, Messenger / biosynthesis
  • Up-Regulation / drug effects

Substances

  • Protein Precursors
  • RNA, Messenger
  • NAD
  • Niacinamide
  • Niacin
  • Glucosephosphate Dehydrogenase
  • Glyceraldehyde-3-Phosphate Dehydrogenases