Abstract
To better understand the role of nicotinic acid and nicotinamide in the regulation of the oxidative stress response, we measured the levels of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and glucose-6-phosphate dehydrogenase (G6PD) mRNA in Jurkat cells treated with these NAD+ precursors. We used a modified nonradioactive Northern blot method and detected the mRNA using 18-mer digoxigenin (DIG)-labeled oligonucleotides as probes. We observed increased levels of the mRNAs for the two enzymes in treated cells. Our findings suggest that the NAD+ precursors may protect against oxidative stress and DNA damage by up-regulating the stress response genes GAPDH and G6PD.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Gene Expression Regulation, Enzymologic / drug effects
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Glucosephosphate Dehydrogenase / biosynthesis*
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Glucosephosphate Dehydrogenase / genetics
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Glyceraldehyde-3-Phosphate Dehydrogenases / biosynthesis*
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
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Humans
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Jurkat Cells
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NAD / chemistry
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NAD / pharmacology
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Niacin / pharmacology*
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Niacinamide / pharmacology*
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Oxidative Stress
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Protein Precursors / chemistry
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Protein Precursors / pharmacology*
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RNA, Messenger / biosynthesis
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Up-Regulation / drug effects
Substances
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Protein Precursors
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RNA, Messenger
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NAD
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Niacinamide
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Niacin
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Glucosephosphate Dehydrogenase
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Glyceraldehyde-3-Phosphate Dehydrogenases