A non-toxic protein (TsNTxP) from Tityus serrulatus scorpion venom has been shown to be an efficient immunogen and anti-TsNTxP antibodies recognize and neutralize the effect of Tityus serrulatus venom [Chávez-Olórtegui et al., 1997. Toxicon 35, 213-221]. With the purpose of studying the organization of the gene that code for this protein, we have isolated a full length cDNA clone for TsNTxP from a cDNA expression library using anti-TsNTxP antibodies. The nucleotide sequence of the gene that encodes TsNTxP was also obtained and it reveals the presence of an intron within the signal peptide sequence. The TsNTxP gene showed high degree of similarity with genes encoding toxins from scorpions of the genus Tityrus.