Abstract
By chemical modification of different lysine residues, benzoylbenzoyl (BzBz) groups were introduced into neurotoxin II Naja naja oxiana (NT-II), a short-chain snake venom alpha-neurotoxin, while p-benzoylphenylalanyl (Bpa) residue was incorporated in the course of peptide synthesis at position 11 of alpha-conotoxin G1, a neurotoxic peptide from marine snails. Although the crosslinking yields for iodinated BzBz derivatives of NT-II and for Bpa analogue of G1 to the membrane-bound Torpedo californica nicotinic acetylcholine receptor (AChR) are relatively low, the subunit labeling patterns confirm the earlier conclusions, derived from arylazide or diazirine photolabels, that alpha-neurotoxins and alpha-conotoxins bind at the subunit interfaces. Detecting the labeled alpha-subunit with iodinated Bpa analogue of G1 provided a direct proof for the contact between this subunit and alpha-conotoxin molecule.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzophenones / chemical synthesis*
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Benzophenones / chemistry
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Benzophenones / metabolism
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Benzophenones / pharmacology
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Binding Sites
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Cobra Neurotoxin Proteins / chemistry*
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Cobra Neurotoxin Proteins / metabolism
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Cobra Neurotoxin Proteins / pharmacology
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Conotoxins*
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Cross-Linking Reagents
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In Vitro Techniques
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Mollusk Venoms / chemistry*
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Mollusk Venoms / metabolism
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Mollusk Venoms / pharmacology
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Peptides, Cyclic / chemistry*
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Peptides, Cyclic / metabolism
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Peptides, Cyclic / pharmacology
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Photoaffinity Labels / chemical synthesis*
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Photoaffinity Labels / chemistry
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Photoaffinity Labels / metabolism
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Photoaffinity Labels / pharmacology
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Protein Conformation
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Receptors, Nicotinic / chemistry
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Receptors, Nicotinic / drug effects*
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Receptors, Nicotinic / metabolism
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Torpedo / metabolism
Substances
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Benzophenones
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Cobra Neurotoxin Proteins
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Conotoxins
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Cross-Linking Reagents
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Mollusk Venoms
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Peptides, Cyclic
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Photoaffinity Labels
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Receptors, Nicotinic
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neurotoxin II, Naja naja oxiana
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conotoxin GI