Single-step purification of immunotoxins containing a high ionic charge ribosome inactivating protein clavin by carboxymethyl high-performance membrane chromatography

J Chromatogr A. 1999 Jan 15;830(2):329-35. doi: 10.1016/s0021-9673(98)00933-9.

Abstract

High-performance membrane chromatography (HPMC) and HPLC hydroxyapatite chromatography were compared for their efficiency in purifying immunotoxins (ITs) containing the ribosome-inactivating protein clavin, which is characterized by a high anionic charge and a low molecular mass. Both methods efficiently removed unreacted clavin from the conjugate crude mixture, but only the cation-exchange HPMC allowed efficient single-step separation of the unreacted monoclonal antibody (mAb) from ITs obtained by different coupling procedures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anions
  • Antibodies, Monoclonal / isolation & purification
  • Cations
  • Chromatography / methods*
  • Chromatography, High Pressure Liquid
  • Fungal Proteins / isolation & purification*
  • Immunotoxins / isolation & purification*
  • Molecular Weight
  • Protein Synthesis Inhibitors / isolation & purification*
  • Ribonucleases*

Substances

  • Anions
  • Antibodies, Monoclonal
  • Cations
  • Fungal Proteins
  • Immunotoxins
  • Protein Synthesis Inhibitors
  • Ribonucleases
  • CLA protein, Aspergillus clavatus