Analysis of 1,5-anhydro-D-fructose, microthecin, and 4-deoxy-glycero-hexo-2,3-diulose in algae using gas chromatography-mass spectrometry in selected ion monitoring mode

Anal Biochem. 1999 Mar 1;268(1):35-42. doi: 10.1006/abio.1998.3013.

Abstract

A sensitive and selective method for detection and quantification of 1,5-anhydro-D-fructose, microthecin, and 4-deoxy-glycero-hexo-2, 3-diulose using GC-MS in selected ion monitoring mode has been developed. These compounds, which occur in many biological systems, have here been quantified in the red alga Gracilariopsis lemaneiformis. A screening of other algae showed the occurrence of 1, 5-anhydro-D-fructose in several other species of red algae as well as in some green and brown algae.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Eukaryota / chemistry*
  • Fructose / analogs & derivatives*
  • Fructose / analysis
  • Gas Chromatography-Mass Spectrometry / methods*
  • Ketoses / analysis*
  • Quality Control
  • Rats

Substances

  • 4-deoxy-glycero-hexo-2,3-diulose
  • Ketoses
  • microthecin
  • Fructose
  • 1,5-anhydrofructose