Toxic mechanisms of nanoplastics exposure at environmental concentrations on juvenile red swamp crayfish (Procambarus clarkii): From multiple perspectives

Long Wang; Qianqian Zhu; Meng Hu; Xinyi Zhou; Tianyu Guan; Nan Wu; Chuankun Zhu; Hui Wang; Guiling Wang; Jiale Li

doi:10.1016/j.envpol.2024.124125

Toxic mechanisms of nanoplastics exposure at environmental concentrations on juvenile red swamp crayfish (Procambarus clarkii): From multiple perspectives

Environ Pollut. 2024 May 11:352:124125. doi: 10.1016/j.envpol.2024.124125. Online ahead of print.

Authors

Long Wang¹, Qianqian Zhu², Meng Hu², Xinyi Zhou², Tianyu Guan¹, Nan Wu³, Chuankun Zhu², Hui Wang⁴, Guiling Wang⁵, Jiale Li⁶

Affiliations

¹ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an, Jiangsu, 223300, China.
² Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China.
³ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China; Jiangsu Engineering Center for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huai'an, 223300, China.
⁴ Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: whh524@sina.com.
⁵ Jiangsu Engineering Center for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huai'an, 223300, China; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an, Jiangsu, 223300, China.
⁶ Jiangsu Engineering Center for Breeding of Special Aquatic Organisms, Huaiyin Normal University, Huai'an, 223300, China; Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Huaiyin Normal University, Huai'an, Jiangsu, 223300, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.

PMID: 38740244
DOI: 10.1016/j.envpol.2024.124125

Abstract

Nanoplastics pollution has emerged as a global issue due to its widespread potential toxicity. This study delved in to toxic effects of nanoplastics on juvenile P. clarkii and molecular mechanisms from perspectives of growth, biochemical, histopathological analysis and transcriptome level for the first time. The findings of this study indicated that nanoplastics of different concentrations have varying influence mechanisms on juvenile P. clarkii. Nanoplastics have inhibitory effects on growth of juvenile P. clarkii, can induce oxidative stress. The biochemical analysis and transcriptome results indicated that 10 mg/L nanoplastics can activate the antioxidant defense system and non-specific immune system in juvenile P. clarkii, and affect energy metabolism and oxidative phosphorylation. While 20 mg/L and 40 mg/L have a destructive influence on the immune function in juvenile P. clarkii, leading to lipid peroxidation and oxidative damage, and induce apoptosis, can affect ion transport and osmotic pressure regulation. The findings of this study can offer foundational data for delving further into impacts of nanoplastics on crustaceans and toxicity mechanism.

Keywords: Molecular mechanism; Nanoplastics; Procambarus clarkii; Toxicity; Transcriptome.