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Affimer proteins are versatile and renewable affinity reagents.
Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, AlQallaf D, Roberts AP, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC. Tiede C, et al. Elife. 2017 Jun 27;6:e24903. doi: 10.7554/eLife.24903. Elife. 2017. PMID: 28654419 Free PMC article.
Alternative reagents to antibodies in imaging applications.
Bedford R, Tiede C, Hughes R, Curd A, McPherson MJ, Peckham M, Tomlinson DC. Bedford R, et al. Among authors: tiede c. Biophys Rev. 2017 Aug;9(4):299-308. doi: 10.1007/s12551-017-0278-2. Epub 2017 Jul 27. Biophys Rev. 2017. PMID: 28752365 Free PMC article. Review.
Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology.
Hughes DJ, Tiede C, Penswick N, Tang AA, Trinh CH, Mandal U, Zajac KZ, Gaule T, Howell G, Edwards TA, Duan J, Feyfant E, McPherson MJ, Tomlinson DC, Whitehouse A. Hughes DJ, et al. Among authors: tiede c. Sci Signal. 2017 Nov 14;10(505):eaaj2005. doi: 10.1126/scisignal.aaj2005. Sci Signal. 2017. PMID: 29138295 Free PMC article.
Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action.
Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, Morgan AW. Robinson JI, et al. Among authors: tiede c. Proc Natl Acad Sci U S A. 2018 Jan 2;115(1):E72-E81. doi: 10.1073/pnas.1707856115. Epub 2017 Dec 15. Proc Natl Acad Sci U S A. 2018. PMID: 29247053 Free PMC article.
45 results