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Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide.
Zumárraga M, Vaz Domínguez C, Camarero S, Shleev S, Polaina J, Martínez-Arias A, Ferrer M, De Lacey AL, Fernández VM, Ballesteros A, Plou FJ, Alcalde M. Zumárraga M, et al. Among authors: plou fj. Comb Chem High Throughput Screen. 2008 Dec;11(10):807-16. doi: 10.2174/138620708786734235. Comb Chem High Throughput Screen. 2008. PMID: 19075602
Production of Galacto-oligosaccharides by the β-Galactosidase from Kluyveromyces lactis : comparative analysis of permeabilized cells versus soluble enzyme.
Rodriguez-Colinas B, de Abreu MA, Fernandez-Arrojo L, de Beer R, Poveda A, Jimenez-Barbero J, Haltrich D, Ballesteros Olmo AO, Fernandez-Lobato M, Plou FJ. Rodriguez-Colinas B, et al. Among authors: plou fj. J Agric Food Chem. 2011 Oct 12;59(19):10477-84. doi: 10.1021/jf2022012. Epub 2011 Sep 8. J Agric Food Chem. 2011. PMID: 21888310
Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im+H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants.
Plou FJ, Kowlessur D, Malthouse JP, Mellor GW, Hartshorn MJ, Pinitglang S, Patel H, Topham CM, Thomas EW, Verma C, Brocklehurst K. Plou FJ, et al. J Mol Biol. 1996 Apr 19;257(5):1088-111. doi: 10.1006/jmbi.1996.0225. J Mol Biol. 1996. PMID: 8632470
103 results