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Aspartic acid 214 in Citrobacter freundii tyrosine phenol-lyase ensures sufficient C--H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site.
Demidkina TV, Faleev NG, Papisova AI, Bazhulina NP, Kulikova VV, Gollnick PD, Phillips RS. Demidkina TV, et al. Among authors: papisova ai. Biochim Biophys Acta. 2006 Jul;1764(7):1268-76. doi: 10.1016/j.bbapap.2006.05.001. Epub 2006 May 16. Biochim Biophys Acta. 2006. PMID: 16793353